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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Effect of sevoflurane on the expression of <t>miRNA</t> in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.
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Image Search Results


Effect of sevoflurane on the expression of miRNA in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.

Journal: OncoTargets and therapy

Article Title: Volatile anesthetic sevoflurane suppresses lung cancer cells and miRNA interference in lung cancer cells

doi: 10.2147/OTT.S171672

Figure Lengend Snippet: Effect of sevoflurane on the expression of miRNA in A549 cell. Notes: Cells were exposed to 3% sevoflurane for 30 minutes, and the relative expressions of miRNA223, 221, 155, 146, 34, and 21 were evaluated by qRT-PCR. Data are shown as the mean ± SD. *Indicates a statistically significant difference ( P <0.05). Abbreviations: qRT-PCR, quantitative real time polymerase chain reaction; SEVO, sevoflurane group.

Article Snippet: Quantitative PCR was carried out using SYBR Premix Ex TaqTM (All-in-OneTM miRNA qPCR Kit; GeneCopoeia, Inc., Rockville, MD, USA) according to the manufacturer’s protocol.

Techniques: Expressing, Quantitative RT-PCR, Real-time Polymerase Chain Reaction